Microscopy

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Microscopy is the technical field of using microscopes to view samples and objects that cannot be seen with the unaided eye (objects that are not within the resolution range of the normal eye). There are three well-known branches of microscopy, optical, electron, and scanning probe microscopy. (Wikipedia.org)






Conferences related to Microscopy

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2020 IEEE 16th International Workshop on Advanced Motion Control (AMC)

AMC2020 is the 16th in a series of biennial international workshops on Advanced Motion Control which aims to bring together researchers from both academia and industry and to promote omnipresent motion control technologies and applications.


2019 20th International Conference on Solid-State Sensors, Actuators and Microsystems & Eurosensors XXXIII (TRANSDUCERS & EUROSENSORS XXXIII)

The world's premiere conference in MEMS sensors, actuators and integrated micro and nano systems welcomes you to attend this four-day event showcasing major technological, scientific and commercial breakthroughs in mechanical, optical, chemical and biological devices and systems using micro and nanotechnology.The major areas of activity in the development of Transducers solicited and expected at this conference include but are not limited to: Bio, Medical, Chemical, and Micro Total Analysis Systems Fabrication and Packaging Mechanical and Physical Sensors Materials and Characterization Design, Simulation and Theory Actuators Optical MEMS RF MEMS Nanotechnology Energy and Power


2019 IEEE 16th International Symposium on Biomedical Imaging (ISBI)

The IEEE International Symposium on Biomedical Imaging (ISBI) is the premier forum for the presentation of technological advances in theoretical and applied biomedical imaging.ISBI 2019 will be the 16th meeting in this series. The previous meetings have played a leading role in facilitating interaction between researchers in medical and biological imaging. The 2019 meeting will continue this tradition of fostering cross fertilization among different imaging communities and contributing to an integrative approach to biomedical imaging across all scales of observation.


2019 IEEE 46th Photovoltaic Specialists Conference (PVSC)

Photovoltaic materials, devices, systems and related science and technology


2019 IEEE 69th Electronic Components and Technology Conference (ECTC)

premier components, packaging and technology conference


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Periodicals related to Microscopy

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Antennas and Propagation, IEEE Transactions on

Experimental and theoretical advances in antennas including design and development, and in the propagation of electromagnetic waves including scattering, diffraction and interaction with continuous media; and applications pertinent to antennas and propagation, such as remote sensing, applied optics, and millimeter and submillimeter wave techniques.


Applied Superconductivity, IEEE Transactions on

Contains articles on the applications and other relevant technology. Electronic applications include analog and digital circuits employing thin films and active devices such as Josephson junctions. Power applications include magnet design as well asmotors, generators, and power transmission


Biomedical Circuits and Systems, IEEE Transactions on

The Transactions on Biomedical Circuits and Systems addresses areas at the crossroads of Circuits and Systems and Life Sciences. The main emphasis is on microelectronic issues in a wide range of applications found in life sciences, physical sciences and engineering. The primary goal of the journal is to bridge the unique scientific and technical activities of the Circuits and Systems ...


Biomedical Engineering, IEEE Reviews in

The IEEE Reviews in Biomedical Engineering will review the state-of-the-art and trends in the emerging field of biomedical engineering. This includes scholarly works, ranging from historic and modern development in biomedical engineering to the life sciences and medicine enabled by technologies covered by the various IEEE societies.


Biomedical Engineering, IEEE Transactions on

Broad coverage of concepts and methods of the physical and engineering sciences applied in biology and medicine, ranging from formalized mathematical theory through experimental science and technological development to practical clinical applications.


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Most published Xplore authors for Microscopy

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Xplore Articles related to Microscopy

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A New Technique in Scanning Near Field Optical Microscopy Used for Investigations on the Biological Samples

2018 20th International Conference on Transparent Optical Networks (ICTON), 2018

We present a new technique based on apertureless near field microscopy using a femtosecond Ti:sapphire laser that is used for the investigations on biological samples. This technique is integrated in a multimodal microscopy system. The beam of a Ti:sapphire laser is focused by using the objective of an inverted microscope on the surface sample. Electric field of the laser beam ...


Fractal analysis correlation of the images from scanning laser microscopy techniques and atomic force microscopy

2017 19th International Conference on Transparent Optical Networks (ICTON), 2017

The laser scanning laser microscopy techniques and atomic force microscopy give complementary information at micro- and nano-scales regarding the surface samples. By using a multimodal microscopy system, having more optical techniques based on far field and near field and an atomic force microscope the same area of the investigated samples. The system is able to acquire the optical images having ...


Nanoscale imaging by using label free microscopy techniques

2017 19th International Conference on Transparent Optical Networks (ICTON), 2017

The objective of our work is connected with label free investigations at nanoscale by using a new multimodal microscopy system by using near field label free techniques. The system includes the techniques with hundred nanometers resolution and with few nanometers resolution, being able to image simultaneously the same sample area giving complementary information. Our system includes also an atomic force ...


Hydroxyapatite surface charge investigated by scanning probe microscopy

2014 16th International Conference on Transparent Optical Networks (ICTON), 2014

It is well known that electrical properties such as local electrostatic charge distribution at biomaterial surface plays a significant role in biological interactions. The methods currently employed in measuring surface charge in biomaterials such as zeta potential or potentiostatic titration, however, can only measure global charge distribution in macroscopic and colloidal state of biomaterials. By using scanning probe microscopy techniques ...


Investigations at nanoscale by using fluorescence in apertureless scanning near field microscopy

2013 15th International Conference on Transparent Optical Networks (ICTON), 2013

Fluorescence apertureless near-field optical microscopy (FASNOM) gained interest from researchers in the last years because of its promising capabilities: sub-wavelength resolution and ability to exploit the chemical sensitivity of fluorescent tags. FASNOM exploits the fact that metallic structures in close proximity of a fluorophore or a photoluminescent sample have complex effects on fluorescence, inducing changes in both fluorescence lifetime and ...


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Educational Resources on Microscopy

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IEEE-USA E-Books

  • A New Technique in Scanning Near Field Optical Microscopy Used for Investigations on the Biological Samples

    We present a new technique based on apertureless near field microscopy using a femtosecond Ti:sapphire laser that is used for the investigations on biological samples. This technique is integrated in a multimodal microscopy system. The beam of a Ti:sapphire laser is focused by using the objective of an inverted microscope on the surface sample. Electric field of the laser beam oscillates in a parallel plan with the sample surface and electric dipoles created by the electric field emit a radiation that is modulated by the AFM tip. This radiation is collected by the objective of the s-SNOM module, placed above the sample being sent to the detector. The images shown a better good contrast compared to those in s-SNOM.

  • Fractal analysis correlation of the images from scanning laser microscopy techniques and atomic force microscopy

    The laser scanning laser microscopy techniques and atomic force microscopy give complementary information at micro- and nano-scales regarding the surface samples. By using a multimodal microscopy system, having more optical techniques based on far field and near field and an atomic force microscope the same area of the investigated samples. The system is able to acquire the optical images having hundred nanometers or nanometers resolutions and surface topography at nanoscale. Fractal analysis is a very useful tool for quantifying and simulating the complex patterns encountered in microscopic images. The objective of this work is to perform a study of the complex optical and morphological features of certain biological tissues by using fractal geometry. It is shown a correlation between optical images and surface morphology regarding fractal geometry and that fractal dimension is a good candidate to quantify the different images.

  • Nanoscale imaging by using label free microscopy techniques

    The objective of our work is connected with label free investigations at nanoscale by using a new multimodal microscopy system by using near field label free techniques. The system includes the techniques with hundred nanometers resolution and with few nanometers resolution, being able to image simultaneously the same sample area giving complementary information. Our system includes also an atomic force microscope. To image the human skin samples has been used two label free techniques working at nanoscale: scattering scanning near optical microscopy(s-SNOM) and second harmonic generation using an apertureless-scanning near optical microscope (SHG-SNOM).

  • Hydroxyapatite surface charge investigated by scanning probe microscopy

    It is well known that electrical properties such as local electrostatic charge distribution at biomaterial surface plays a significant role in biological interactions. The methods currently employed in measuring surface charge in biomaterials such as zeta potential or potentiostatic titration, however, can only measure global charge distribution in macroscopic and colloidal state of biomaterials. By using scanning probe microscopy techniques we investigated the surface charge domains placed on the hydroxyapatite surface. Scattering near field optical microscopy (s-SNOM) was used to image electrostatic charge trapped at pre-existing and irradiation induced defects within these domains, while phase imaging in atomic force microscopy was used to image the carbon contamination. The two techniques can be used for nanoscale imaging.

  • Investigations at nanoscale by using fluorescence in apertureless scanning near field microscopy

    Fluorescence apertureless near-field optical microscopy (FASNOM) gained interest from researchers in the last years because of its promising capabilities: sub-wavelength resolution and ability to exploit the chemical sensitivity of fluorescent tags. FASNOM exploits the fact that metallic structures in close proximity of a fluorophore or a photoluminescent sample have complex effects on fluorescence, inducing changes in both fluorescence lifetime and emission intensity. The detection concept of FASNOM is based on the periodic perturbation of the interaction between a fluorescent sample and the excitation beam by using a sharp probe. The aim of the present work is to present the tip enhanced near field fluorescence and topography characterization of collagen by using an atomic force microscope upgraded with an apertureless microscope.

  • Nanoscale Investigations of Optical Fiber by Using Scattering Scanning Near-Field Optical Microscopy

    Scattering Scanning Near-field Optical Microscopy (s-SNOM) is proposed as a powerful tool for quantitative analysis of cross-sectional area of optical fibers. The s-SNOM images are processed pixel-by-pixel in order to map the refractive index of the optical fibers' cross-sectional surface. s-SNOM imaging technique is widely known for its capability to reach nanoscale resolution, therefore the obtained maps of refractive index based on s-SNOM data are as well characterized by nanoscale resolution. Combined with Atomic Force Microscopy (AFM) images simultaneously acquired with s-SNOM, this technique proves to be a powerful tool not only for characterization of refractive index profile of optical fibers, but also for quality check of optical fibers (or other types of optical waveguides) at nanoscale. Particularly, in this study we prove the capability of s-SNOM to map the refractive index of cross-sectional area of a Panda-style polarization- maintaining single mode optical fiber.

  • Photonic-Corral-Mode Quantum Ring Lasers investigated by Laser Scanning Microscopy and Near Field Microscopy

    Ultralow threshold microcavity lasers are ideal candidates for high-density optical interconnect light sources. Although they have been extensively studied for the last decade, muA-level electro-pumped quantum confined lasers are still under investigation. Photonic-corral-mode quantum ring (PQR) lasers with the linewidth narrower than 0.55 generate micro-to-nano-ampere thresholds, and become ideal for CMOS-driven high-density emitter arrays for intra-chip optical interconnect as well as other applications such as displays. By using laser beam induced current technique (LBIC) technique in laser scanning microscopy (LSM) we investigated the photocurrent confinement in the laser structure. The photocurrent map gives the possibility to analyze the laser structure uniformity. The emission spectra of a PQR laser was investigated by using of microscopic optical fiber probe.

  • Integration of scanning near field polarization optical microscopy and atomic force microscopy for investigation of magnetic and ferroelectric materials

    We demonstrate application of integration scanning near-field optical microscopy and atomic force microscopy for investigation of magnetic and ferroelectric materials. This integration is made by using innovative optical and micromechanical components. Optical measuring head with cantilever holder was designed for both near-field optical microscopy and atomic force microscopy modes. Hollow pyramid cantilever with aperture at the tip was designed for registration both optical signal and tip=sample interaction forces. Cantilever aperture was made by ion beam at the center of pyramid. Same probe was used for both topography and domain structure visualization of magnetic samples. Bi: YIG-thin films topography and domain structure were investigated by this method as well as by conventional magnetic force microscopy (MFM). In integrated method of scanning nearfield polarization optical microscopy and atomic force microscopy samples were excited through cantilever aperture by tightly focused linearly polarized laser beam. Magneto- optical effect of the rotation of polarization plane of transmitted light depends on domain orientation. Visualization of magnetic domains was performed by detecting cross polarized component of transmitted light. Near field map of magnetic domains provides direct information of the magnetic moment of domains unlike MFM image which provides distribution of magnetic force gradient. Moreover, non-magnetic hollow-pyramid probe prevents sample from possible disturbances by internal magnetic moment of the MFM probe. Comparison of results obtained by near field polarization microscopy and conventional MFM was performed. We demonstrate possibility of investigation of topography and domain structure ferroelectric materials by integration method of near-field optical microscopy and atomic force microscopy.

  • Bag-of-features approaches for combined classification of laser scanning microscopy and spectroscopy data sets

    The Bag-of-Features (BoF) paradigm represents a solid solution for the automated classification of digital images. Several BoF approaches for classification of microscopy data have been reported in the past decade, but their number is very low considering the potential that BoF methods hold with respect to this subject. In this contribution we discuss strategies for using BoF architectures for the automated classification of 1D and 2D data sets collected using Laser Scanning Microscopy techniques.

  • Image fusion for photonic quantum ring laser structures investigated by confocal scanning laser microscopy

    Confocal Scanning Laser Microscopy (CSLM) allows the acquisition of image stacks, representing optical sections on the volume of the specimen. An image corresponding to an optical section will in some cases contain defocused, low contrast or saturated regions for the areas of the sample which are not in focal plane. In the case of the Photonic Quantum Ring (PQR) laser structures, it is important to have images consisting of regions of uniform quality in order to observe morphological details that are linked to the photocurrent confinement. In this purpose we have developed an image fusion method, which based on a stack of CSLM images will output a fused image consisting of square regions from different images in the stack. In this paper we present the image fusion algorithm that we have used and the results that we have obtained on PQR laser structures.



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